› 1p4t

X-ray diffraction
2.55Å resolution

Crystal structure of Neisserial surface protein A (NspA)

Released:
DOI: 10.2210/pdb1p4t/pdb
PDB entry 1p4t coloured by chain, front view.
PDB entry 1p4t coloured by chain, side view.
PDB entry 1p4t coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Crystal structure of Neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential.
Vandeputte-Rutten L, Bos MP, Tommassen J, Gros P
J Biol Chem 278 24825-30 (2003)
PMID: 12716881

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-193217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin opacity type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 16.58 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RP17 (Residues: 20-174; Coverage: 100%)
Gene name: nspA
Sequence domains: Opacity family porin protein
Structure domains: Porin

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand CXE 5 x CXE
Ligand ETA 1 x ETA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: R32
Unit cell:
a: 97.372Å b: 97.372Å c: 171.939Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.225 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Molecular basis of bacterial outer membrane permeability revisited.
Nikaido H. (2003)
14 more

28 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
27 more