PDBe 1p4n

X-ray diffraction
1.9Å resolution

Crystal Structure of Weissella viridescens FemX:UDP-MurNAc-pentapeptide complex

Released:

Function and Biology Details

Reaction catalysed:
L-alanyl-tRNA(Ala) + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L- lysyl-D-alanyl-D-alanine = tRNA(Ala) + UDP-N-acetyl-alpha-D-muramoyl-L- alanyl-D-glutamyl-N(6)-(L-alanyl)-L-lysyl-D-alanyl-D-alanine. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
UDP-N-acetylmuramoylpentapeptide-lysine N(6)-alanyltransferase Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 38.2 KDa
Source organism: Weissella viridescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EY50 (Residues: 2-336; Coverage: 100%)
Gene name: femX
Sequence domains: FemAB family
Structure domains: Aminopeptidase
UDP-MurNAc-pentapeptide Chain: B
Molecule details ›
Chain: B
Length: 5 amino acids
Theoretical weight: 1.15 KDa
Source organism: Staphylococcus aureus

Ligands and Environments

2 bound ligands:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 42.201Å b: 100.61Å c: 46.538Å
α: 90° β: 115.64° γ: 90°
R-values:
R R work R free
0.185 0.172 0.207
Expression system: Escherichia coli