› 1p3j

X-ray diffraction
1.9Å resolution

Adenylate Kinase from Bacillus subtilis

Released:
DOI: 10.2210/pdb1p3j/pdb
PDB entry 1p3j coloured by chain, front view.
PDB entry 1p3j coloured by chain, side view.
PDB entry 1p3j coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structures and analysis of highly homologous psychrophilic, mesophilic, and thermophilic adenylate kinases.
Bae E, Phillips GN
J Biol Chem 279 28202-8 (2004)
PMID: 15100224

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 24.15 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16304 (Residues: 1-217; Coverage: 100%)
Gene names: BSU01370, adk
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 1 x MG
Ligand AP5 1 x AP5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P21
Unit cell:
a: 32.8Å b: 73.2Å c: 38.1Å
α: 90° β: 101.6° γ: 90°
R-values:
R R work R free
0.197 0.197 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Cold-adapted enzymes.
Siddiqui et al. (2006)
11 more

10 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
9 more