PDBe 1p22

X-ray diffraction
2.95Å resolution

Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity on the SCFbeta-TrCP1 ubiquitin ligase

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
S-phase kinase-associated protein 1 Chain: B
Molecule details ›
Chain: B
Length: 145 amino acids
Theoretical weight: 16.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Best match: P63208 (Residues: 1-163)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Catenin beta-1 Chain: C
Molecule details ›
Chain: C
Length: 26 amino acids
Theoretical weight: 2.9 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Best match: P35222 (Residues: 19-44)
Gene names: CTNNB, CTNNB1, OK/SW-cl.35, PRO2286
F-box/WD repeat-containing protein 1A Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 49.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Best match: Q9Y297 (Residues: 175-605)
Gene names: BTRC, BTRCP, FBW1A, FBXW1A

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P31
Unit cell:
a: 82.6Å b: 82.6Å c: 111.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.23 0.286
Expression system: Spodoptera frugiperda