PDBe 1ozh

X-ray diffraction
2Å resolution

The crystal structure of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetolactate synthase, catabolic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 566 amino acids
Theoretical weight: 61.36 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27696 (Residues: 1-559; Coverage: 100%)
Gene names: budB, ilvK
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P1
Unit cell:
a: 86.32Å b: 92.814Å c: 97.42Å
α: 67.97° β: 63.48° γ: 67.68°
R-values:
R R work R free
0.195 0.191 0.228
Expression system: Escherichia coli BL21(DE3)