Function and Biology

OLD YELLOW ENZYME AT 2 ANGSTROMS RESOLUTION: OVERALL STRUCTURE, LIGAND BINDING AND COMPARISON WITH RELATED FLAVOPROTEINS

Source organism: Saccharomyces pastorianus
Biochemical function: catalytic activity
Biological process: oxidation-reduction process
Cellular component: not assigned

EC 1.6.99.1: NADPH dehydrogenase

Reaction catalysed:
NADPH + acceptor = NADP(+) + reduced acceptor.
Comments:
  • FMN in yeasts; FAD in plants.
Systematic name:
NADPH:acceptor oxidoreductase
Alternative Name(s):
  • NADPH diaphorase
  • NADPH-dehydrogenase
  • NADPH-diaphorase
  • NADPH(2) diaphorase
  • NADPH(2)-dehydrogenase
  • OYE
  • TPNH dehydrogenase
  • TPNH-diaphorase
  • Diaphorase
  • Dihydronicotinamide adenine dinucleotide phosphate dehydrogenase
  • Old yellow enzyme
  • Reduced nicotinamide adenine dinucleotide phosphate dehydrogenase
  • Triphosphopyridine diaphorase
  • Triphosphopyridine nucleotide diaphorase
  • NADPH:(acceptor) oxidoreductase

Sequence family

Pfam Protein family (Pfam)
PF00724
Domain description: NADH:flavin oxidoreductase / NADH oxidase family
Occurring in:
  1. NADPH dehydrogenase 1
1 copy of Pfam domain PF00724 (NADH:flavin oxidoreductase / NADH oxidase family) in NADPH dehydrogenase 1 in PDB 1oya.

InterPro InterPro annotations
IPR001155
Domain description: NADH:flavin oxidoreductase/NADH oxidase, N-terminal
Occurring in:
  1. NADPH dehydrogenase 1
IPR013785
Domain description: Aldolase-type TIM barrel
Occurring in:
  1. NADPH dehydrogenase 1

Structure domain

CATH CATH domain
3.20.20.70
Class: Alpha Beta
Architecture: Alpha-Beta Barrel
Topology: TIM Barrel
Homology: Aldolase class I
Occurring in:
  1. NADPH dehydrogenase 1
1 copy of CATH domain 3.20.20.70 (TIM Barrel) in NADPH dehydrogenase 1 in PDB 1oya.
SCOP SCOP annotation
51396
Class: Alpha and beta proteins (a/b)
Fold: TIM beta/alpha-barrel
Superfamily: FMN-linked oxidoreductases
Occurring in:
  1. NADPH dehydrogenase 1
1 copy of SCOP domain 51396 (FMN-linked oxidoreductases) in NADPH dehydrogenase 1 in PDB 1oya.