PDBe 1otg

X-ray diffraction
2.1Å resolution

5-CARBOXYMETHYL-2-HYDROXYMUCONATE ISOMERASE

Released:

Function and Biology Details

Reaction catalysed:
5-carboxymethyl-2-hydroxymuconate = (3E,5R)-5-carboxy-2-oxohept-3- enedioate. 
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-carboxymethyl-2-hydroxymuconate Delta-isomerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 125 amino acids
Theoretical weight: 14.16 KDa
Source organism: Escherichia coli C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05354 (Residues: 2-126; Coverage: 99%)
Gene name: hpcD
Sequence domains: 5-carboxymethyl-2-hydroxymuconate isomerase
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P41212
Unit cell:
a: 90.3Å b: 90.3Å c: 129.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 not available not available
Expression system: Escherichia coli