PDBe 1ot7

X-ray diffraction
2.9Å resolution

Structural Basis for 3-deoxy-CDCA Binding and Activation of FXR

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bile acid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.75 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q62735 (Residues: 240-468; Coverage: 49%)
Gene names: Bar, Fxr, Nr1h4, Rip14
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
dodecamer peptide fragment of RPGR-interacting protein 1 Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 12 amino acids
Theoretical weight: 1.46 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 99.536Å b: 107.129Å c: 69.203Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.279
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided