PDBe 1osv

X-ray diffraction
2.5Å resolution

STRUCTURAL BASIS FOR BILE ACID BINDING AND ACTIVATION OF THE NUCLEAR RECEPTOR FXR

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero trimer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bile acid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.85 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q62735 (Residues: 240-469; Coverage: 49%)
Gene names: Bar, Fxr, Nr1h4, Rip14
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 12 amino acids
Theoretical weight: 1.46 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q61026 (Residues: 741-752; Coverage: 1%)
Gene names: Grip1, Ncoa2, Src2, Tif2

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21212
Unit cell:
a: 98.97Å b: 108.518Å c: 69.464Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.242 0.276
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided