PDBe 1osp

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF OUTER SURFACE PROTEIN A OF BORRELIA BURGDORFERI COMPLEXED WITH A MURINE MONOCLONAL ANTIBODY FAB

Released:
Primary publication:
Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab.
Proc. Natl. Acad. Sci. U.S.A. 94 3584-9 (1997)
PMID: 9108020

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig kappa chain C region Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.69 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-106; Coverage: 100%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig gamma-2B chain C region Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.45 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01867 (Residues: 1-97; Coverage: 24%)
Gene name: Igh-3
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Outer surface protein A Chain: O
Molecule details ›
Chain: O
Length: 257 amino acids
Theoretical weight: 27.68 KDa
Source organism: Borrelia burgdorferi B31
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CL66 (Residues: 17-273; Coverage: 100%)
Gene names: BB_A15, ospA
Sequence domains: Borrelia lipoprotein
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 89.1Å b: 91.7Å c: 100.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.295
Expression system: Escherichia coli