› 1oql

X-ray diffraction
3Å resolution

Mistletoe Lectin I from Viscum album complexed with galactose

Released:
DOI: 10.2210/pdb1oql/pdb
PDB entry 1oql coloured by chain, front view.
PDB entry 1oql coloured by chain, side view.
PDB entry 1oql coloured by chain, top view.
Source organism: Viscum album
Primary publication:
Crystal structure at 3 A of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose.
Niwa H, Tonevitsky AG, Agapov II, Saward S, Pfüller U, Palmer RA
Eur J Biochem 270 2739-49 (2003)
PMID: 12823544

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160520 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-galactoside-specific lectin 1 chain A isoform 1 Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 27.87 KDa
Source organism: Viscum album
UniProt:
  • Canonical: P81446 (Residues: 34-282; Coverage: 47%)
Sequence domains: Ribosome inactivating protein
Structure domains:
Beta-galactoside-specific lectin 1 chain B Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 28.83 KDa
Source organism: Viscum album
UniProt:
  • Canonical: P81446 (Residues: 302-564; Coverage: 50%)
Sequence domains: Ricin-type beta-trefoil lectin domain
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand GAL 2 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P6522
Unit cell:
a: 107.65Å b: 107.65Å c: 311.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.24

Quick links

Citations

2 review citations

Biological activities of ribosome-inactivating proteins and their possible applications as antimicrobial, anticancer, and anti-pest agents and in neuroscience research.
Akkouh et al. (2015)
1 more

7 mentions without citation

Plant-Derived Lectins as Potential Cancer Therapeutics and Diagnostic Tools.
Mazalovska et al. (2020)
6 more