1oo3 Experiments and Validation

Solution NMR

Source organism: Bos taurus
Refinement method: distance geometry, simulated annealing
Number of deposited models: 1 model

Sample information

Author description:
P395S mutant of the p85 regulatory subunit of the N-terminal src homology 2 domain of PI3-Kinase
Source organism: Bos taurus
Expression system: Escherichia coli

Validation information

 
Structure ensemble information
Refinement method: distance geometry, simulated annealing
Number of deposited models: 1

Experimental information

Sample 1
Contents: 1.5mM P395S-15N,13C, 0.1M KCl Recorded spectra: 3D_13C-separated_NOESY, 3D_15N-separated_NOESY, CC(CO)NH, HCC(CO)NH, HN(CO)CA, HNCA-J

Samples and spectra
NMR spectrometers used
Bruker AMX 500 MHz, Bruker DMX 600 MHz, Bruker DRX 800 MHz