› 1ojt

X-ray diffraction
2.75Å resolution

STRUCTURE OF DIHYDROLIPOAMIDE DEHYDROGENASE

Released:
DOI: 10.2210/pdb1ojt/pdb
PDB entry 1ojt coloured by chain, front view.
PDB entry 1ojt coloured by chain, side view.
PDB entry 1ojt coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Molecular structure of the lipoamide dehydrogenase domain of a surface antigen from Neisseria meningitidis.
Li de la Sierra I, Pernot L, Prangé T, Saludjian P, Schiltz M, Fourme R, Padrón G
J Mol Biol 269 129-41 (1997)
PMID: 9193005

Function and Biology Details

Reaction catalysed: 
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175910 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyl dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 50.74 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51225 (Residues: 112-593; Coverage: 81%)
Gene name: m-6
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE
Spacegroup: P43212
Unit cell:
a: 138.05Å b: 138.05Å c: 79.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 not available 0.232
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions.
Perham RN. (2000)
2 more

5 mentions without citation

Identifying the subproteome of kinetically stable proteins via diagonal 2D SDS/PAGE.
Xia et al. (2007)
4 more