PDBe 1ohh

X-ray diffraction
2.8Å resolution

BOVINE MITOCHONDRIAL F1-ATPASE complexed with the inhibitor protein IF1

Released:
Source organism: Bos taurus

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
ATPase inhibitor, mitochondrial Chain: H
Molecule details ›
Chain: H
Length: 84 amino acids
Theoretical weight: 9.6 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01096 (Residues: 26-109; Coverage: 77%)
Gene names: ATPI, ATPIF1
ATP synthase subunit beta, mitochondrial Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 482 amino acids
Theoretical weight: 51.76 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00829 (Residues: 47-528; Coverage: 91%)
Gene name: ATP5B
Sequence domains:
Structure domains:
ATP synthase subunit alpha, mitochondrial Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 510 amino acids
Theoretical weight: 55.3 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P19483 (Residues: 44-553; Coverage: 92%)
Gene names: ATP5A1, ATP5A2
Sequence domains:
Structure domains:
ATP synthase subunit gamma, mitochondrial Chain: G
Molecule details ›
Chain: G
Length: 272 amino acids
Theoretical weight: 30.19 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05631 (Residues: 26-297; Coverage: 91%)
Gene names: ATP5C, ATP5C1
Sequence domains: ATP synthase
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 272.3Å b: 107.2Å c: 152.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.28
Expression system: Escherichia coli