PDBe 1oh9

X-ray diffraction
1.91Å resolution

Acetylglutamate kinase from Escherichia coli complexed with MgADP, N-acetyl-L-glutamate and the transition-state mimic AlF4-

Released:

Function and Biology Details

Reaction catalysed:
ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylglutamate kinase Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 27.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6C8 (Residues: 1-258; Coverage: 100%)
Gene names: JW5553, argB, b3959
Sequence domains: Amino acid kinase family
Structure domains: Carbamate kinase

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2221
Unit cell:
a: 59.457Å b: 72.22Å c: 107.214Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.229
Expression system: Escherichia coli BL21(DE3)