PDBe 1ogy

X-ray diffraction
3.2Å resolution

Crystal structure of the heterodimeric nitrate reductase from Rhodobacter sphaeroides

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Periplasmic nitrate reductase Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 802 amino acids
Theoretical weight: 90.11 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: Q53176 (Residues: 30-831; Coverage: 100%)
Gene names: RHOS4_40230, RSP_4116, napA
Sequence domains:
Structure domains:
Periplasmic nitrate reductase, electron transfer subunit Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 130 amino acids
Theoretical weight: 14.59 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Rhodobacter sphaeroides
UniProt:
  • Canonical: Q53177 (Residues: 25-154; Coverage: 100%)
Gene names: RHOS4_40240, RSP_4117, napB

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 123Å b: 225.2Å c: 154.6Å
α: 90° β: 92.1° γ: 90°
R-values:
R R work R free
0.25 0.25 0.269
Expression system: Rhodobacter sphaeroides