PDBe 1odt

X-ray diffraction
1.7Å resolution

cephalosporin C deacetylase mutated, in complex with acetate

Released:

Function and Biology Details

Reactions catalysed:
Cephalosporin C + H(2)O = deacetylcephalosporin C + acetate. 
Deacetylation of xylans and xylo-oligosaccharides. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cephalosporin-C deacetylase Chains: C, H
Molecule details ›
Chains: C, H
Length: 318 amino acids
Theoretical weight: 35.83 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P94388 (Residues: 1-318; Coverage: 100%)
Gene names: BSU03180, cah
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R32
Unit cell:
a: 157.167Å b: 157.17Å c: 132.098Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.156 0.191
Expression system: Escherichia coli BL21(DE3)