› 1o9p

X-ray diffraction
1.8Å resolution

Crystal structure of the S131A mutant of Malonamidase E2 complexed with malonate from Bradyrhizobium japonicum

Released:
DOI: 10.2210/pdb1o9p/pdb
PDB entry 1o9p coloured by chain, front view.
PDB entry 1o9p coloured by chain, side view.
PDB entry 1o9p coloured by chain, top view.
Source organism: Bradyrhizobium japonicum
Primary publication:
Characterization of a novel Ser-cisSer-Lys catalytic triad in comparison with the classical Ser-His-Asp triad.
Shin S, Yun YS, Koo HM, Kim YS, Choi KY, Oh BH
J Biol Chem 278 24937-43 (2003)
PMID: 12711609

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 414 amino acids
Theoretical weight: 43.72 KDa
Source organism: Bradyrhizobium japonicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZIV5 (Residues: 1-414; Coverage: 100%)
Sequence domains: Amidase
Structure domains: Amidase signature (AS) domain

Ligands and Environments

1 bound ligand:
Ligand MLA 2 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21212
Unit cell:
a: 103.581Å b: 94.79Å c: 75.065Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Structure and function of fatty acid amide hydrolase.
McKinney et al. (2005)
4 more