PDBe 1o4x

Solution NMR

TERNARY COMPLEX OF THE DNA BINDING DOMAINS OF THE OCT1 AND SOX2 TRANSCRIPTION FACTORS WITH A 19MER OLIGONUCLEOTIDE FROM THE HOXB1 REGULATORY ELEMENT

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription factor SOX-2 Chain: B
Molecule details ›
Chain: B
Length: 88 amino acids
Theoretical weight: 10.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P48431 (Residues: 39-121; Coverage: 26%)
Gene name: SOX2
Sequence domains: HMG (high mobility group) box
Structure domains: DNA Binding (I), subunit A
POU domain, class 2, transcription factor 1 Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 19.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14859 (Residues: 280-442; Coverage: 22%)
Gene names: OCT1, OTF1, POU2F1
Sequence domains:
Structure domains:
5'-D(*CP*AP*TP*TP*AP*GP*CP*AP*TP*GP*AP*CP*AP*AP*AP*GP*AP*CP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.83 KDa
5'-D(*TP*GP*TP*CP*TP*TP*TP*GP*TP*CP*AP*TP*GP*CP*TP*AP*AP*TP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.82 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: CONJOINED RIGID BODY/TORSION ANGLE DYNAMICS
Expression system: Escherichia coli