PDBe 1o01

X-ray diffraction
2.15Å resolution

Human mitochondrial aldehyde dehydrogenase complexed with crotonaldehyde, NAD(H) and Mg2+

Released:
Source organism: Homo sapiens
Primary publication:
Coenzyme isomerization is integral to catalysis in aldehyde dehydrogenase.
Biochemistry 42 7100-9 (2003)
PMID: 12795606

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase, mitochondrial Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 500 amino acids
Theoretical weight: 54.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P05091 (Residues: 18-517; Coverage: 97%)
Gene names: ALDH2, ALDM
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 142.133Å b: 150.571Å c: 176.782Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.17 0.206
Expression system: Escherichia coli BL21