PDBe 1nzv

X-ray diffraction
2.1Å resolution

Crystal Structure of Src SH2 domain bound to doubly phosphorylated peptide PQpYIpYVPA


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Doubly phosphorylated peptide PQpYIpYVPA Chain: C
Molecule details ›
Chain: C
Length: 8 amino acids
Theoretical weight: 1.11 KDa
Source organism: Synthetic construct
Expression system: Not provided
Tyrosine-protein kinase transforming protein Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.84 KDa
Source organism: Rous sarcoma virus (strain Schmidt-Ruppin)
Expression system: Escherichia coli BL21
  • Canonical: P00524 (Residues: 145-247; Coverage: 20%)
Gene name: V-SRC
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 100.256Å b: 67.727Å c: 29.636Å
α: 90° β: 90° γ: 90°
R R work R free
0.224 0.224 0.271
Expression systems:
  • Not provided
  • Escherichia coli BL21