PDBe 1nwq

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF C/EBPALPHA-DNA COMPLEX

Released:

Function and Biology Details

Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
CCAAT/enhancer-binding protein alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 62 amino acids
Theoretical weight: 7.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05554 (Residues: 281-340; Coverage: 17%)
Gene name: Cebpa
Sequence domains: Basic region leucine zipper
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
5'-D(*AP*AP*AP*CP*TP*GP*GP*AP*TP*TP*GP*CP*GP*CP*AP*AP*TP*AP*GP*GP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.52 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*TP*TP*CP*CP*TP*AP*TP*TP*GP*CP*GP*CP*AP*AP*TP*CP*CP*AP*GP*TP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.36 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21212
Unit cell:
a: 140.892Å b: 53.089Å c: 67.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.275
Expression systems:
  • Escherichia coli
  • Not provided