PDBe 1nsy

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF NH3-DEPENDENT NAD+ SYNTHETASE FROM BACILLUS SUBTILIS

Released:

Function and Biology Details

Reaction catalysed:
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 30.3 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P08164 (Residues: 2-272; Coverage: 100%)
Gene names: BSU03130, nadE, outB
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 53.3Å b: 87.8Å c: 61.4Å
α: 90° β: 110.6° γ: 90°
R-values:
R R work R free
0.174 0.174 0.23