PDBe 1nsi

X-ray diffraction
2.55Å resolution

HUMAN INDUCIBLE NITRIC OXIDE SYNTHASE, ZN-BOUND, L-ARG COMPLEX

Released:

Function and Biology Details

Reaction catalysed:
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, inducible Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 431 amino acids
Theoretical weight: 49.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35228 (Residues: 74-504; Coverage: 37%)
Gene names: NOS2, NOS2A
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P43212
Unit cell:
a: 187.35Å b: 187.35Å c: 227.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.243
Expression system: Escherichia coli