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Crystal structure of galactose mutarotase from Lactococcus lactis mutant E304Q complexed with galactose

X-ray diffraction
1.85Å resolution
PDB entry 1ns0 coloured by chain, front view.
PDB entry 1ns0 coloured by chain, side view.
PDB entry 1ns0 coloured by chain, top view.
Source organism: Lactococcus lactis
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Aldose 1-epimerase
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Multimeric state: homo dimer
Accessible surface area: 25789.39 Å2
Buried surface area: 2810.05 Å2
Dissociation area: 1,003.91 Å2
Dissociation energy (ΔGdiss): 11.67 kcal/mol
Dissociation entropy (TΔSdiss): 12.51 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-195605

Macromolecules

Aldose 1-epimerase
Chains: A, B
Length: 347 amino acids
Theoretical weight: 38.62 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZB17 (Residues: 1-339; Coverage: 100%)
Gene name: galM
Pfam: Aldose 1-epimerase
InterPro:
CATH: Beta-galactosidase; Chain A, domain 5
SCOP: Aldose 1-epimerase (mutarotase)
Aldose 1-epimerase in PDB entry 1ns0, assembly 1, front view.
Aldose 1-epimerase in PDB entry 1ns0, assembly 1, side view.
Aldose 1-epimerase in PDB entry 1ns0, assembly 1, top view.
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