PDBe 1npq

Solution NMR

structure of a rhodamine-labeled N-domain Troponin C mutant (Ca2+ saturated) in complex with skeletal Troponin I 115-131

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Troponin C, skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 9.93 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02588 (Residues: 2-91; Coverage: 55%)
Gene name: TNNC2
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Troponin I, fast skeletal muscle Chain: B
Molecule details ›
Chain: B
Length: 17 amino acids
Theoretical weight: 1.89 KDa
Source organism: Oryctolagus cuniculus
Expression system: Not provided
UniProt:
  • Canonical: P02643 (Residues: 116-132; Coverage: 9%)
Gene name: TNNI2

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 74%
Refinement method: simulated annealing using torsion angle dynamics AND cartesian dynamics with the program CNS 1.1
Chemical shifts: BMR5738  
Expression systems:
  • Escherichia coli
  • Not provided