PDBe 1np0

X-ray diffraction
2.5Å resolution

Human lysosomal beta-hexosaminidase isoform B in complex with intermediate analogue NAG-thiazoline

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero 12-mer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-hexosaminidase subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 58 amino acids
Theoretical weight: 6.42 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07686 (Residues: 50-107; Coverage: 11%)
Gene names: HCC7, HEXB
Beta-hexosaminidase subunit beta chain A Chains: D, F
Molecule details ›
Chains: D, F
Length: 241 amino acids
Theoretical weight: 27.82 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07686 (Residues: 316-556; Coverage: 47%)
Gene names: HCC7, HEXB
Beta-hexosaminidase subunit beta chain B Chains: C, E
Molecule details ›
Chains: C, E
Length: 190 amino acids
Theoretical weight: 21.65 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07686 (Residues: 122-311; Coverage: 37%)
Gene names: HCC7, HEXB

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6122
Unit cell:
a: 112.414Å b: 112.414Å c: 397.232Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.198 0.227