PDBe 1nnr

X-ray diffraction
2.25Å resolution

Crystal structure of a probable fosfomycin resistance protein (PA1129) from Pseudomonas aeruginosa with sulfate present in the active site

Released:

Function and Biology Details

Reaction catalysed:
RX + glutathione = HX + R-S-glutathione. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione transferase FosA Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.14 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I4K6 (Residues: 1-135; Coverage: 100%)
Gene names: PA1129, fosA
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200H
Spacegroup: P212121
Unit cell:
a: 44.93Å b: 64.524Å c: 78.825Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.268
Expression system: Escherichia coli BL21(DE3)