PDBe 1nma

X-ray diffraction
3Å resolution

N9 NEURAMINIDASE COMPLEXES WITH ANTIBODIES NC41 AND NC10: EMPIRICAL FREE-ENERGY CALCULATIONS CAPTURE SPECIFICITY TRENDS OBSERVED WITH MUTANT BINDING DATA

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FAB NC10 Chain: L
Molecule details ›
Chain: L
Length: 109 amino acids
Theoretical weight: 12.13 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
FAB NC10 Chain: H
Molecule details ›
Chain: H
Length: 122 amino acids
Theoretical weight: 13.42 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Neuraminidase Chain: N
Molecule details ›
Chain: N
Length: 388 amino acids
Theoretical weight: 43.64 KDa
Source organism: Influenza A virus
UniProt:
  • Canonical: P05803 (Residues: 83-470; Coverage: 83%)
Gene name: NA
Structure domains: Neuraminidase

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I422
Unit cell:
a: 171.5Å b: 171.5Å c: 160.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 not available