› 1nlz

X-ray diffraction
3Å resolution

Crystal structure of unliganded traffic ATPase of the type IV secretion system of helicobacter pylori

Released:
DOI: 10.2210/pdb1nlz/pdb
PDB entry 1nlz coloured by chain, front view.
PDB entry 1nlz coloured by chain, side view.
PDB entry 1nlz coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretion.
Savvides SN, Yeo HJ, Beck MR, Blaesing F, Lurz R, Lanka E, Buhrdorf R, Fischer W, Haas R, Waksman G
EMBO J 22 1969-80 (2003)
PMID: 12727865

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial type II secretion system protein E domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 330 amino acids
Theoretical weight: 37.91 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BK04 (Residues: 1-330; Coverage: 100%)
Gene names: ACM37_01770, C2R45_06065, C2R60_04995, C2R64_07520, C2R66_03525, C2R72_03110, C2S01_07015, C2S26_06255, CV728_03100, D8X81_02825, EC515_06990, EC547_07075, EC572_06310, EC594_00565, EDB76_02000, F7219_04320, F7220_05215, HP0525, SE88_02715, cag-alfa, cag-alpha, cagAlpha, cagalpha, virB11
Sequence domains: Type II/IV secretion system protein
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P31
Unit cell:
a: 104.53Å b: 104.53Å c: 168.72Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.239 0.298
Expression system: Escherichia coli

Quick links

Citations

50 review citations

Mobile genetic elements: the agents of open source evolution.
Frost et al. (2005)
49 more

7 mentions without citation

Recent advances in the structural and molecular biology of type IV secretion systems.
Trokter et al. (2014)
6 more