PDBe 1nkk

X-ray diffraction
2.6Å resolution

COMPLEX STRUCTURE OF HCMV PROTEASE AND A PEPTIDOMIMETIC INHIBITOR

Released:

Function and Biology Details

Reaction catalysed:
Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Assemblin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 256 amino acids
Theoretical weight: 28.16 KDa
Source organism: Human betaherpesvirus 5
Expression system: Escherichia coli
UniProt:
  • Canonical: P16753 (Residues: 1-256; Coverage: 36%)
Gene names: APNG, UL80
Structure domains: Herpes virus serine proteinase, assemblin
Peptidomimetic inhibitor Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 6 amino acids
Theoretical weight: 552 Da
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21212
Unit cell:
a: 105.17Å b: 215.25Å c: 52.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.28
Expression systems:
  • Escherichia coli
  • Not provided