PDBe 1ni4

X-ray diffraction
1.95Å resolution

HUMAN PYRUVATE DEHYDROGENASE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial Chains: B, D
Molecule details ›
Chains: B, D
Length: 341 amino acids
Theoretical weight: 37.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11177 (Residues: 31-359; Coverage: 92%)
Gene names: PDHB, PHE1B
Sequence domains:
Structure domains:
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial Chains: A, C
Molecule details ›
Chains: A, C
Length: 365 amino acids
Theoretical weight: 41.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08559 (Residues: 30-390; Coverage: 93%)
Gene names: PDHA1, PHE1A
Sequence domains: Dehydrogenase E1 component
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 64.337Å b: 126.889Å c: 190.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.244
Expression system: Escherichia coli