PDBe 1nfi

X-ray diffraction
2.7Å resolution

I-KAPPA-B-ALPHA/NF-KAPPA-B COMPLEX

Released:
Source organism: Homo sapiens
Primary publication:
Structure of an IkappaBalpha/NF-kappaB complex.
Cell 95 749-58 (1998)
PMID: 9865693

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nuclear factor NF-kappa-B p50 subunit Chains: B, D
Molecule details ›
Chains: B, D
Length: 107 amino acids
Theoretical weight: 12.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19838 (Residues: 248-354; Coverage: 11%)
Gene name: NFKB1
Sequence domains: Rel homology dimerisation domain
Structure domains: Immunoglobulins
Transcription factor p65 Chains: A, C
Molecule details ›
Chains: A, C
Length: 301 amino acids
Theoretical weight: 34.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04206 (Residues: 20-320; Coverage: 55%)
Gene names: NFKB3, RELA
Sequence domains:
Structure domains:
NF-kappa-B inhibitor alpha Chains: E, F
Molecule details ›
Chains: E, F
Length: 213 amino acids
Theoretical weight: 23.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25963 (Residues: 70-282; Coverage: 67%)
Gene names: IKBA, MAD3, NFKBI, NFKBIA
Sequence domains: Ankyrin repeat
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 49.094Å b: 91.08Å c: 190.98Å
α: 90° β: 96.32° γ: 90°
R-values:
R R work R free
0.223 0.223 0.265
Expression system: Escherichia coli