PDBe 1nf9

X-ray diffraction
1.5Å resolution

Crystal Structure of PhzD protein from Pseudomonas aeruginosa

Released:

Function and Biology Details

Reaction catalysed:
Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenazine biosynthesis protein PhzD Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.22 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7DC80 (Residues: 1-207; Coverage: 100%)
Gene names: PA1902, PA4213, phzD1, phzD2
Sequence domains: Isochorismatase family
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2221
Unit cell:
a: 68.85Å b: 77.03Å c: 82.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.225
Expression system: Escherichia coli BL21(DE3)