PDBe 1nex

X-ray diffraction
2.7Å resolution

Crystal Structure of ScSkp1-ScCdc4-CPD peptide complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cell division control protein 4 Chains: B, D
Molecule details ›
Chains: B, D
Length: 464 amino acids
Theoretical weight: 53.14 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P07834 (Residues: 263-744; Coverage: 59%)
Gene names: CDC4, YFL009W
Sequence domains:
Structure domains:
GLL(TPO)PPQSG Chains: E, F
Molecule details ›
Chains: E, F
Length: 9 amino acids
Theoretical weight: 949 Da
Suppressor of kinetochore protein 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 169 amino acids
Theoretical weight: 19.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P52286 (Residues: 1-194; Coverage: 86%)
Gene names: CBF3D, D9798.14, SKP1, YDR328C
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D, APS BEAMLINE 14-BM-C
Spacegroup: P32
Unit cell:
a: 107.669Å b: 107.669Å c: 168.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.239 0.273
Expression system: Escherichia coli