PDBe 1ndo

X-ray diffraction
2.25Å resolution

NAPHTHALENE 1,2-DIOXYGENASE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Naphthalene 1,2-dioxygenase system, large oxygenase component Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 449 amino acids
Theoretical weight: 49.66 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A110 (Residues: 1-449; Coverage: 100%)
Gene names: nahA3, nahAC, ndoB, ndoC2
Sequence domains:
Structure domains:
Naphthalene 1,2-dioxygenase system, small oxygenase component Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 194 amino acids
Theoretical weight: 22.97 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A112 (Residues: 1-194; Coverage: 100%)
Gene names: nahAD, ndoC
Sequence domains: Ring hydroxylating beta subunit
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I222
Unit cell:
a: 105Å b: 174Å c: 282.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.19 0.24
Expression system: Escherichia coli