PDBe 1ncb

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURES OF TWO MUTANT NEURAMINIDASE-ANTIBODY COMPLEXES WITH AMINO ACID SUBSTITUTIONS IN THE INTERFACE

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero 12-mer (preferred)
hetero 20-mer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig gamma-2A chain C region, membrane-bound form Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.67 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01865 (Residues: 1-100; Coverage: 25%)
Gene name: Igh-1a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
IGG2A-KAPPA NC41 FAB (LIGHT CHAIN) Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.72 KDa
Source organism: Mus musculus
UniProt:
Structure domains: Immunoglobulins
Neuraminidase Chain: N
Molecule details ›
Chain: N
Length: 389 amino acids
Theoretical weight: 43.84 KDa
Source organism: Influenza A virus (A/tern/Australia/G70C/1975(H11N9))
UniProt:
  • Canonical: P03472 (Residues: 82-470; Coverage: 83%)
Gene name: NA
Structure domains: Neuraminidase

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P4212
Unit cell:
a: 167Å b: 167Å c: 124Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.165 not available