PDBe 1nba

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE ANALYSIS, REFINEMENT AND ENZYMATIC REACTION MECHANISM OF N-CARBAMOYLSARCOSINE AMIDOHYDROLASE FROM ARTHROBACTER SP. AT 2.0 ANGSTROMS RESOLUTION

Released:

Function and Biology Details

Reaction catalysed:
N-carbamoylsarcosine + H(2)O = sarcosine + CO(2) + NH(3). 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-carbamoylsarcosine amidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 264 amino acids
Theoretical weight: 29.08 KDa
Source organism: Arthrobacter sp.
Expression system: Not provided
UniProt:
  • Canonical: P32400 (Residues: 1-264; Coverage: 100%)
Sequence domains: Isochorismatase family
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 136.22Å b: 122.29Å c: 70.87Å
α: 90° β: 91.82° γ: 90°
R-values:
R R work R free
0.186 0.186 not available
Expression system: Not provided