PDBe 1n8z

X-ray diffraction
2.52Å resolution

Crystal structure of extracellular domain of human HER2 complexed with Herceptin Fab

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Herceptin Fab (antibody) - heavy chain Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 23.43 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Herceptin Fab (antibody) - light chain Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.47 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Receptor tyrosine-protein kinase erbB-2 Chain: C
Molecule details ›
Chain: C
Length: 607 amino acids
Theoretical weight: 66.99 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04626 (Residues: 23-629; Coverage: 49%)
Gene names: ERBB2, HER2, MLN19, NEU, NGL
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 66.17Å b: 109.77Å c: 175.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.222 0.284
Expression systems:
  • Not provided
  • Cricetulus griseus