Structure analysis

Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin

X-ray diffraction
3.35Å resolution
Source organism: Thermus thermophilus
Assembly composition:
hetero 23-mer (preferred)
Entry contents: 20 distinct polypeptide molecules
3 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 23-mer
Accessible surface area: 283855.86 Å2
Buried surface area: 103876.52 Å2
Dissociation area: 1,592.67 Å2
Dissociation energy (ΔGdiss): 14.85 kcal/mol
Dissociation entropy (TΔSdiss): 12.7 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-215885

Macromolecules

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Chain: L
Length: 135 amino acids
Theoretical weight: 14.92 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:
CATH: Nucleic acid-binding proteins
SCOP: Cold shock DNA-binding domain-like

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Chain: O
Length: 88 amino acids
Theoretical weight: 10.45 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SJ76 (Residues: 2-89; Coverage: 99%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
InterPro:
CATH: S15/NS1, RNA-binding
SCOP: Ribosomal protein S15

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Chain: P
Length: 88 amino acids
Theoretical weight: 10.41 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
InterPro:
CATH: S16 Ribosomal Protein; Chain: A;
SCOP: Ribosomal protein S16

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Chain: R
Length: 88 amino acids
Theoretical weight: 10.24 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
InterPro:
CATH: Ribosomal protein S18
SCOP: Ribosomal protein S18

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Chain: T
Length: 106 amino acids
Theoretical weight: 11.74 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80380 (Residues: 1-106; Coverage: 100%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
InterPro:
CATH: Ribosomal protein S20
SCOP: Ribosomal protein S20

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Name: 16S ribosomal RNA
Representative chains: A
Length: 1522 nucleotides
Theoretical weight: 493.96 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: ANTICODON STEM-LOOP OF SER TRANSFER RNA
Representative chains: Y
Length: 17 nucleotides
Theoretical weight: 5.48 KDa

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Name: A-SITE MESSENGER RNA FRAGMENT
Representative chains: Z
Length: 6 nucleotides
Theoretical weight: 1.79 KDa

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