PDBe 1n2d

X-ray diffraction
2Å resolution

Ternary complex of MLC1P bound to IQ2 and IQ3 of Myo2p, a class V myosin

Released:
Primary publication:
Structure of the light chain-binding domain of myosin V.
Proc. Natl. Acad. Sci. U.S.A. 102 12718-23 (2005)
PMID: 16120677

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Myosin light chain 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 16.33 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53141 (Residues: 2-149; Coverage: 99%)
Gene names: G3080, MLC1, YGL106W
Structure domains: EF-hand
Myosin-2 Chain: C
Molecule details ›
Chain: C
Length: 48 amino acids
Theoretical weight: 5.54 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P19524 (Residues: 806-853; Coverage: 3%)
Gene names: CDC66, MYO2, O6167, YOR326W
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21212
Unit cell:
a: 80.017Å b: 64.24Å c: 72.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided