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NITROGENASE COMPLEX FROM AZOTOBACTER VINELANDII STABILIZED BY ADP-TETRAFLUOROALUMINATE

Source organism: Azotobacter vinelandii
Biochemical function: iron-sulfur cluster binding
Biological process: nitrogen fixation
Cellular component: molybdenum-iron nitrogenase complex

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EC 1.18.6.1: Nitrogenase

Reaction catalysed:
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
Systematic name:
Ferredoxin:dinitrogen oxidoreductase (ATP-hydrolyzing, molybdenum-dependent)
Alternative Name(s):
  • Reduced ferredoxin:dinitrogen oxidoreductase (ATP-hydrolyzing)

GO terms

Sequence families

Pfam Protein families (Pfam)
PF00142
Domain description: 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family
Occurring in:
  1. Nitrogenase iron protein 1
The deposited structure of PDB entry 1n2c contains 4 copies of Pfam domain PF00142 (4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family) in Nitrogenase iron protein 1. Showing 1 copy in chain E.

PF00148
Domain description: Nitrogenase component 1 type Oxidoreductase
Occurring in:
  1. Nitrogenase molybdenum-iron protein alpha chain
  2. Nitrogenase molybdenum-iron protein beta chain
The deposited structure of PDB entry 1n2c contains 2 copies of Pfam domain PF00148 (Nitrogenase component 1 type Oxidoreductase) in Nitrogenase molybdenum-iron protein alpha chain. Showing 1 copy in chain A.
The deposited structure of PDB entry 1n2c contains 2 copies of Pfam domain PF00148 (Nitrogenase component 1 type Oxidoreductase) in Nitrogenase molybdenum-iron protein beta chain. Showing 1 copy in chain B.

PF11844
Domain description: Domain of unknown function (DUF3364)
Occurring in:
  1. Nitrogenase molybdenum-iron protein beta chain
The deposited structure of PDB entry 1n2c contains 2 copies of Pfam domain PF11844 (Domain of unknown function (DUF3364)) in Nitrogenase molybdenum-iron protein beta chain. Showing 1 copy in chain B.

InterPro InterPro annotations
IPR000392
Domain description: NifH/frxC family
Occurring in:
  1. Nitrogenase iron protein 1
IPR030655
Domain description: NifH/chlL conserved site
Occurring in:
  1. Nitrogenase iron protein 1
IPR005977
Domain description: Nitrogenase iron protein NifH
Occurring in:
  1. Nitrogenase iron protein 1
IPR027417
Domain description: P-loop containing nucleoside triphosphate hydrolase
Occurring in:
  1. Nitrogenase iron protein 1
IPR000318
Domain description: Nitrogenase component 1, conserved site
Occurring in:
  1. Nitrogenase molybdenum-iron protein alpha chain
  2. Nitrogenase molybdenum-iron protein beta chain
IPR000510
Domain description: Nitrogenase/oxidoreductase, component 1
Occurring in:
  1. Nitrogenase molybdenum-iron protein alpha chain
  2. Nitrogenase molybdenum-iron protein beta chain
IPR005972
Domain description: Nitrogenase molybdenum-iron protein alpha chain
Occurring in:
  1. Nitrogenase molybdenum-iron protein alpha chain
IPR010143
Domain description: Nitrogenase component 1, alpha chain
Occurring in:
  1. Nitrogenase molybdenum-iron protein alpha chain
IPR024564
Domain description: Nitrogenase molybdenum-iron protein beta chain, N-terminal
Occurring in:
  1. Nitrogenase molybdenum-iron protein beta chain
IPR005976
Domain description: Nitrogenase molybdenum-iron protein beta chain
Occurring in:
  1. Nitrogenase molybdenum-iron protein beta chain

Structure domains

CATH CATH domains
3.40.50.1980
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: Nitrogenase molybdenum iron protein domain
Occurring in:
  1. Nitrogenase molybdenum-iron protein alpha chain
  2. Nitrogenase molybdenum-iron protein beta chain
The deposited structure of PDB entry 1n2c contains 6 copies of CATH domain 3.40.50.1980 (Rossmann fold) in Nitrogenase molybdenum-iron protein alpha chain. Showing 3 copies in chain A.
The deposited structure of PDB entry 1n2c contains 6 copies of CATH domain 3.40.50.1980 (Rossmann fold) in Nitrogenase molybdenum-iron protein beta chain. Showing 3 copies in chain B.
3.40.50.300
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: P-loop containing nucleotide triphosphate hydrolases
Occurring in:
  1. Nitrogenase iron protein 1
The deposited structure of PDB entry 1n2c contains 4 copies of CATH domain 3.40.50.300 (Rossmann fold) in Nitrogenase iron protein 1. Showing 1 copy in chain E.
1.20.89.10
Class: Mainly Alpha
Architecture: Up-down Bundle
Topology: Nitrogenase Molybdenum-iron Protein, subunit B; domain 4
Homology: Nitrogenase Molybdenum-iron Protein, subunit B, domain 4
Occurring in:
  1. Nitrogenase molybdenum-iron protein beta chain
The deposited structure of PDB entry 1n2c contains 2 copies of CATH domain 1.20.89.10 (Nitrogenase Molybdenum-iron Protein, subunit B; domain 4) in Nitrogenase molybdenum-iron protein beta chain. Showing 1 copy in chain B.
SCOP SCOP annotations
52652
Class: Alpha and beta proteins (a/b)
Fold: P-loop containing nucleoside triphosphate hydrolases
Superfamily: P-loop containing nucleoside triphosphate hydrolases
Occurring in:
  1. Nitrogenase iron protein 1
The deposited structure of PDB entry 1n2c contains 4 copies of SCOP domain 52652 (Nitrogenase iron protein-like) in Nitrogenase iron protein 1. Showing 1 copy in chain E.
53816
Class: Alpha and beta proteins (a/b)
Fold: Chelatase-like
Superfamily: "Helical backbone" metal receptor
Occurring in:
  1. Nitrogenase molybdenum-iron protein alpha chain
  2. Nitrogenase molybdenum-iron protein beta chain
The deposited structure of PDB entry 1n2c contains 2 copies of SCOP domain 53816 (Nitrogenase iron-molybdenum protein) in Nitrogenase molybdenum-iron protein alpha chain. Showing 1 copy in chain A.
The deposited structure of PDB entry 1n2c contains 2 copies of SCOP domain 53816 (Nitrogenase iron-molybdenum protein) in Nitrogenase molybdenum-iron protein beta chain. Showing 1 copy in chain B.

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