PDBe 1n0w

X-ray diffraction
1.7Å resolution

Crystal structure of a RAD51-BRCA2 BRC repeat complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
peptide linker Chain: L
Molecule details ›
Chain: L
Length: 14 amino acids
Theoretical weight: 1.23 KDa
UniProt:
ARTIFICIAL GLY-SER-MSE-GLY PEPTIDE Chain: C
Molecule details ›
Chain: C
Length: 4 amino acids
Theoretical weight: 397 Da
UniProt:
DNA repair protein RAD51 homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 26.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06609 (Residues: 97-339; Coverage: 72%)
Gene names: RAD51, RAD51A, RECA
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Breast cancer type 2 susceptibility protein Chain: B
Molecule details ›
Chain: B
Length: 35 amino acids
Theoretical weight: 3.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51587 (Residues: 1517-1551; Coverage: 1%)
Gene names: BRCA2, FACD, FANCD1
Sequence domains: BRCA2 repeat

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 57.3Å b: 59.14Å c: 77.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.206
Expression system: Escherichia coli BL21(DE3)