› 1myw

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF A YELLOW FLUORESCENT PROTEIN WITH IMPROVED MATURATION AND REDUCED ENVIRONMENTAL SENSITIVITY

Released:
DOI: 10.2210/pdb1myw/pdb
PDB entry 1myw coloured by chain, front view.
PDB entry 1myw coloured by chain, side view.
PDB entry 1myw coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity.
Rekas A, Alattia JR, Nagai T, Miyawaki A, Ikura M
J Biol Chem 277 50573-8 (2002)
PMID: 12370172

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.88 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR2 1 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3112
Unit cell:
a: 82.704Å b: 82.704Å c: 72.555Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.218 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.
Klionsky et al. (2008)
13 more

24 mentions without citation

Advances in imaging of understudied ions in signaling: A focus on magnesium.
Lazarou et al. (2020)
23 more