PDBe 1mxg

X-ray diffraction
1.6Å resolution

Crystal Strucutre of a (Ca,Zn)-dependent alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei in complex with acarbose

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha amylase Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 50.23 KDa
Source organism: Pyrococcus woesei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7LYT7 (Residues: 26-460; Coverage: 95%)
Gene name: amyA
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 51.48Å b: 76.507Å c: 136.455Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 0.172
Expression system: Escherichia coli BL21(DE3)