PDBe 1mxe

X-ray diffraction
1.7Å resolution

Structure of the Complex of Calmodulin with the Target Sequence of CaMKI

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 16.69 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62152 (Residues: 2-149; Coverage: 99%)
Gene names: CG8472, Cam
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Calcium/calmodulin-dependent protein kinase type 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 25 amino acids
Theoretical weight: 3 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: Q63450 (Residues: 294-318; Coverage: 7%)
Gene name: Camk1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 65.712Å b: 69.581Å c: 75.344Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.229
Expression systems:
  • Escherichia coli BL21
  • Not provided