PDBe 1mus

X-ray diffraction
1.9Å resolution

crystal structure of Tn5 transposase complexed with resolved outside end DNA

Released:
Primary publication:
Structure/function insights into Tn5 transposition.
Curr. Opin. Struct. Biol. 14 50-7 (2004)
PMID: 15102449

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposase for transposon Tn5 Chain: A
Molecule details ›
Chain: A
Length: 477 amino acids
Theoretical weight: 53.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46731 (Residues: 1-476; Coverage: 100%)
  • Best match: Q46731-2 (Residues: 1-421)
Gene names: tnp, tnpA
Sequence domains:
Structure domains:
DNA transferred strand Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.21 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA non-transferred strand Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.05 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P6522
Unit cell:
a: 112.7Å b: 112.7Å c: 235.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.249
Expression systems:
  • Escherichia coli
  • Not provided