Function and Biology

ON THE ROLE OF LYSINE 166 IN THE MECHANISM OF MANDELATE RACEMASE FROM PSEUDOMONAS PUTIDA: MECHANISTIC AND CRYSTALLOGRAPHIC EVIDENCE FOR STEREOSPECIFIC ALKYLATION BY (R)-ALPHA-PHENYLGLYCIDATE

Source organism: Pseudomonas putida
Biochemical function: metal ion binding
Biological process: cellular amino acid catabolic process
Cellular component: not assigned

EC 5.1.2.2: Mandelate racemase

Reaction catalysed:
(S)-mandelate = (R)-mandelate.
Systematic name:
Mandelate racemase

Sequence families

Pfam Protein families (Pfam)
PF13378
Domain description: Enolase C-terminal domain-like
Occurring in:
  1. Mandelate racemase
1 copy of Pfam domain PF13378 (Enolase C-terminal domain-like) in Mandelate racemase in PDB 1mns.

PF02746
Domain description: Mandelate racemase / muconate lactonizing enzyme, N-terminal domain
Occurring in:
  1. Mandelate racemase
1 copy of Pfam domain PF02746 (Mandelate racemase / muconate lactonizing enzyme, N-terminal domain) in Mandelate racemase in PDB 1mns.

InterPro InterPro annotations
IPR036849
Domain description: Enolase-like, C-terminal
Occurring in:
  1. Mandelate racemase
IPR029017
Domain description: Enolase-like, N-terminal
Occurring in:
  1. Mandelate racemase
IPR018110
Domain description: Mandelate racemase/muconate lactonizing enzyme, conserved site
Occurring in:
  1. Mandelate racemase
IPR013342
Domain description: Mandelate racemase/muconate lactonizing enzyme, C-terminal
Occurring in:
  1. Mandelate racemase
IPR029065
Domain description: Enolase C-terminal domain-like
Occurring in:
  1. Mandelate racemase
IPR013341
Domain description: Mandelate racemase/muconate lactonizing enzyme, N-terminal domain
Occurring in:
  1. Mandelate racemase
IPR034593
Domain description: Mandelate racemase-like
Occurring in:
  1. Mandelate racemase
IPR034390
Domain description: Enolase-like superfamily
Occurring in:
  1. Mandelate racemase
IPR015654
Domain description: Mandelate racemase
Occurring in:
  1. Mandelate racemase

Structure domains

CATH CATH domains
3.30.390.10
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Enolase-like; domain 1
Homology: Enolase-like, N-terminal domain
Occurring in:
  1. Mandelate racemase
1 copy of CATH domain 3.30.390.10 (Enolase-like; domain 1) in Mandelate racemase in PDB 1mns.
3.20.20.120
Class: Alpha Beta
Architecture: Alpha-Beta Barrel
Topology: TIM Barrel
Homology: Enolase superfamily
Occurring in:
  1. Mandelate racemase
1 copy of CATH domain 3.20.20.120 (TIM Barrel) in Mandelate racemase in PDB 1mns.
SCOP SCOP annotations
54827
Class: Alpha and beta proteins (a+b)
Fold: Enolase N-terminal domain-like
Superfamily: Enolase N-terminal domain-like
Occurring in:
  1. Mandelate racemase
1 copy of SCOP domain 54827 (Enolase N-terminal domain-like) in Mandelate racemase in PDB 1mns.
51609
Class: Alpha and beta proteins (a/b)
Fold: TIM beta/alpha-barrel
Superfamily: Enolase C-terminal domain-like
Occurring in:
  1. Mandelate racemase
1 copy of SCOP domain 51609 (D-glucarate dehydratase-like) in Mandelate racemase in PDB 1mns.