PDBe 1mnm

X-ray diffraction
2.25Å resolution

YEAST MATALPHA2/MCM1/DNA TERNARY TRANSCRIPTION COMPLEX CRYSTAL STRUCTURE

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the yeast MATalpha2/MCM1/DNA ternary complex.
Nature 391 660-6 (1998)
PMID: 9490409

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Pheromone receptor transcription factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 100 amino acids
Theoretical weight: 11.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P11746 (Residues: 1-100; Coverage: 35%)
Gene names: FUN80, MCM1, YM9532.08, YMR043W
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: SRF-like
Mating-type protein ALPHA2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 87 amino acids
Theoretical weight: 10.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CY08 (Residues: 103-189; Coverage: 41%)
Gene names: ALPHA-2, MAT2A, MATAL2, MATALPHA2, YCR039C, YCR39C
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
DNA (STE6 OPERATOR DNA) Chain: E
Molecule details ›
Chain: E
Length: 26 nucleotides
Theoretical weight: 8.02 KDa
DNA (STE6 OPERATOR DNA) Chain: F
Molecule details ›
Chain: F
Length: 26 nucleotides
Theoretical weight: 7.95 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 70.62Å b: 72.55Å c: 150.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.285
Expression system: Escherichia coli