PDBe 1mm8

X-ray diffraction
2.8Å resolution

Crystal structure of Tn5 Transposase complexed with ME DNA

Released:
Source organism: Escherichia coli
Primary publication:
Evidence for "unseen" transposase--DNA contacts.
J. Mol. Biol. 322 971-82 (2002)
PMID: 12367522

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposase for transposon Tn5 Chain: A
Molecule details ›
Chain: A
Length: 481 amino acids
Theoretical weight: 53.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46731 (Residues: 1-476; Coverage: 100%)
  • Best match: Q46731-2 (Residues: 1-421)
Gene names: tnp, tnpA
Sequence domains:
Structure domains:
ME DNA transferred strand Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.27 KDa
Source organism: Escherichia coli
Expression system: Not provided
ME DNA non-transferred strand Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 5.99 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 112.5Å b: 112.5Å c: 233.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.271
Expression systems:
  • Escherichia coli
  • Not provided